In vitro effects of bisphenol F on antioxidant system indicators in the isolated hepatocytes of rainbow trout (Oncorhyncus mykiss)

Bisphenol F (BPF) has been used frequently in the plastics industry and production of daily consumer products as an alternative to bisphenol A (BPA). It was aimed herein determine cytotoxic effects BPF on hepatocytes isolated from liver rainbow trout (Oncorhyncus mykiss) using lactate dehydrogenase (LDH) assay antioxidant defence system indicators. The cultured were exposed seven concentrations (0, 15.63, 31.25, 62.50, 125, 250, 500 µM) for 24 h. According LDH assay, percentage cytotoxicity increased dose dependently cells. malondialdehyde content, which is indicative lipid peroxidation, significantly at between 15.63 250 µM, whereas it remained unchanged with a concentration µM. activities superoxide dismutase increased, while those catalase decreased all concentrations. Elevated levels reduced glutathione content determined but Significant increases peroxidase found treated 31.25 GST activity only results showed that toxic mechanism mainly based cell membrane damage oxidative stress, have influence defences. Therefore, should be reconsidered safe instead BPA manufacturing industrial or products.